製品の概要

  • 製品名Anti-Spindly antibody
    Spindly 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Spindly
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length Human CCDC99 protein (AAH12568, 1 a.a. ~ 606 a.a) with tag.

  • ポジティブ・コントロール
    • Recombinant tagged full length human CCDC99.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
    • 配列類似性Belongs to the Spindly family.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AA409762 antibody
      • Arsenite related gene 1 protein antibody
      • Arsenite-related gene 1 protein antibody
      • CCDC 99 antibody
      • Ccdc99 antibody
      • Coiled coil domain containing 99 antibody
      • Coiled-coil domain-containing protein 99 antibody
      • FLJ20364 antibody
      • FLJ40690 antibody
      • hSpindly antibody
      • MGC124977 antibody
      • Protein Spindly antibody
      • rhabdomyosarcoma antigen MU RMS 40.4A antibody
      • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
      • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
      • SPDL1 antibody
      • SPDLY_HUMAN antibody
      • Spindle apparatus coiled coil domain containing protein 1 antibody
      • Spindle apparatus coiled coil protein 1 antibody
      • Spindly antibody
      • Spindly homolog antibody
      see all

    Anti-Spindly antibody 画像

    • Anti-Spindly antibody (ab76670) at 1 µg/ml + Recombinant tagged full length human CCDC99 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 70 kDa
      Observed band size : 93 kDa (why is the actual band size different from the predicted?)

    Anti-Spindly antibody (ab76670) 使用論文

    ab76670 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76670.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"