製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

ターゲット情報

  • 機能
    Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
  • 組織特異性
    Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • ドメイン
    The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.
  • 細胞内局在
    Cytoplasm and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SPI B antibody
    • Spi B transcription factor antibody
    • Spib antibody
    • SPIB_HUMAN antibody
    • Transcription factor Spi-B antibody
    see all

画像

  • Anti-SPIB antibody (ab42436) at 3 µg/ml + spleen tissue lysate

    Predicted band size : 29 kDa

プロトコール

参考文献

This product has been referenced in:
  • Wilkinson AC  et al. RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction. Cell Rep 3:116-27 (2013). WB ; Human . Read more (PubMed: 23352661) »

See 1 Publication for this product

レビューと Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Daudi cell line)
Total protein in input
1e+007 cells
Immuno-precipitation step
Protein A/G
Specification
Daudi cell line
Username

Dr. Keijo Viiri

Verified customer

投稿 Aug 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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