製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

ターゲット情報

  • 機能Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
  • 組織特異性Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • ドメインThe protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.
  • 細胞内局在Cytoplasm and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SPI B antibody
    • Spi B transcription factor antibody
    • Spib antibody
    • SPIB_HUMAN antibody
    • Transcription factor Spi-B antibody
    see all

Anti-SPIB antibody 画像

  • All lanes : Anti-SPIB antibody (ab42436) at 0.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Daudi cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SPIB antibody (ab42436) at 0.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-SPIB antibody (ab42436) 使用論文

This product has been referenced in:
  • Wilkinson AC  et al. RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction. Cell Rep 3:116-27 (2013). WB ; Human . Read more (PubMed: 23352661) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab42436.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"