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Our Abpromise guarantee covers the use of ab71700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with SPHK1 peptide (ab187382).
There are two known isoforms of this protein, one predicted around 43 kDa and the other around 51 kDa.
This image is a courtesy of Anonymous Abreview.
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical staining of SPHK1 in Human hepatocarcinoma tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71700 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.