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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SPHK1.
(Peptide available as ab16634.)
Our Abpromise guarantee covers the use of ab16491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.Can be blocked with Human SPHK1 peptide (ab16634).
A collaborator showed positive reactivity when using IMR-32 (human), Raw264.7 (mouse), and PC12 (rat) samples. For further information, please refer abreview.
|ICC/IF||Use a concentration of 5 µg/ml.|
ab16491 detects a band of ~ 100kDa in Human skeletal muscle lysate which is quenched by the blocking peptide (ab16634). The expected band size for Human SPHK1 is ~ 49kDa, therefore the 100kDa band that is detected may represent a dimer. No bands were detected in Human brain, liver or heart lysate.
ab16491 staining human smooth muscle, mouse lung, and rat heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.