製品の概要

  • 製品名Anti-SPHK1 antibody
    SPHK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SPHK1
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SPHK1.

    (Peptide available as ab16634.)

  • ポジティブ・コントロール
    • This antibody gave positive signal for Western Blot in Human skeletal muscle. This antibody gave a positive signal for Immunofluorescence in HepG2 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab16491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.Can be blocked with Human SPHK1 peptide (ab16634).
A collaborator showed positive reactivity when using IMR-32 (human), Raw264.7 (mouse), and PC12 (rat) samples. For further information, please refer abreview.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-SPHK1 antibody 画像

  • All lanes : Anti-SPHK1 antibody (ab16491) at 1 µg/ml

    Lane 1 : Human Skeletal muscle lysate
    Lane 2 : Human Skeletal muscle lysate with Human SPHK1 peptide (ab16634) at 1 µg/ml

    Lysates/proteins at 20 µg/ml per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Predicted band size : 49 kDa

    ab16491 detects a band of ~ 100kDa in Human skeletal muscle lysate which is quenched by the blocking peptide (ab16634). The expected band size for Human SPHK1 is ~ 49kDa, therefore the 100kDa band that is detected may represent a dimer. No bands were detected in Human brain, liver or heart lysate.

     

  • ab16491 staining human smooth muscle, mouse lung, and rat heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab16491 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16491, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SPHK1 antibody (ab16491) 使用論文

This product has been referenced in:
  • Alfonso J  et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26446219) »
  • Martin JL  et al. Inhibition of insulin-like growth factor-binding protein-3 signaling through sphingosine kinase-1 sensitizes triple-negative breast cancer cells to EGF receptor blockade. Mol Cancer Ther 13:316-28 (2014). Human . Read more (PubMed: 24337110) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 17 2014

Thank you for contacting us.

Wegot the data over the IHC-P from a cooperator from us, and haven't validated the sensitivity our self. However, we do have the blocking peptide available if you want to use this to validate this for your experi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human (smooth muscle), mouse (lung), rat (heart))
Specification human (smooth muscle), mouse (lung), rat (heart)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step sequential peroxidase and protein block prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL-60, IMR-32, RAW264.7, PC12)
Loading amount 20 µg
Specification HL-60, IMR-32, RAW264.7, PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 07 2008

Thank you for your enquiry. Antibody ab16491 against human SPHK1 has been raised against a small peptide from amino acids 371 to 384 of this protein. I have done an alignment search to SPHK2 for you, and there is very limited homology between the two. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"