製品の概要

  • 製品名Anti-Sphingomyelin Synthase 2 antibody
    Sphingomyelin Synthase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sphingomyelin Synthase 2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (

    FPLEWWKTGIAFIYAVFNLVLTTVMITVVHERVPPKELSPPLPDKFFDYI

    ) of Human Sphingomyelin Synthase 2 (NP_689834)

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth.
  • 組織特異性Brain, heart, kidney, liver, muscle and stomach.
  • 配列類似性Belongs to the sphingomyelin synthase family.
  • 翻訳後修飾Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization.
  • 細胞内局在Cell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC26963 antibody
    • Phosphatidylcholine:ceramide cholinephosphotransferase 2 antibody
    • SGMS 2 antibody
    • Sgms2 antibody
    • SM synthase antibody
    • SMS 2 antibody
    • SMS2 antibody
    • SMS2_HUMAN antibody
    • Sphingomyelin synthase 2 antibody
    see all

Anti-Sphingomyelin Synthase 2 antibody 画像

  • Anti-Sphingomyelin Synthase 2 antibody (ab87214) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-Sphingomyelin Synthase 2 antibody (ab87214) 使用論文

This product has been referenced in:
  • Yachi R  et al. Subcellular localization of sphingomyelin revealed by two toxin-based probes in mammalian cells. Genes Cells 17:720-7 (2012). WB ; African Green Monkey . Read more (PubMed: 22747662) »

See 1 Publication for this product

Product Wall

The difference is between the immunogens. Both were generated against 14aa length peptides from the region of 72 - 121aa but the peptides differ. Again apologies for the long wait.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"