製品の概要

  • 製品名Anti-SPEN antibody
  • 製品の詳細
    Rabbit polyclonal to SPEN
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
    • 細胞内局在Nuclear. Associates with chromatin.
    • 参照データベース
    • 別名
      • MINT antibody
      • Nuclear receptor transcription cofactor antibody
      • RBM15C antibody
      • SHARP antibody
      • SPEN homolog antibody
      • Spen homolog transcriptional regulator antibody
      see all

    Anti-SPEN antibody 画像

    • Immunoprecipitation/ Western Blot of SPEN.
      Lane 1: ab72266 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72266 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 3 minutes.

    Anti-SPEN antibody (ab72266) 使用論文

    ab72266 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your interest in ab72266. The immunogen for this antibody is 86% homologous with the mouse SPEN protien (UniProt: Q62504). Based on this information, there is a good chance that ab72266 will detect mouse SPEN, but this has not b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"