製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SPC25
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 79-128 (KDNLLKLIAE VKGKKQELEV LTANIQDLKE EYSRKKETIS TANKANAERL) of Human SPC25 (NP_065726).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • 配列類似性
    Belongs to the SPC25 family.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AD024 antibody
    • hSpc25 antibody
    • Kinetochore protein Spc25 antibody
    • SPBC25 antibody
    • SPC25 antibody
    • SPC25_HUMAN antibody
    • spindle pole body component 25 homolog antibody
    see all

画像

  • Anti-SPC25 antibody (ab116328) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 26 kDa

プロトコール

参考文献

ab116328 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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