Anti-SPC24 抗体 [EPR11548(B)] (ab169786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11548(B)] to SPC24
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SPC24 antibody [EPR11548(B)]
SPC24 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11548(B)] to SPC24 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, 293T, MOLT4, 293 and TF1 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11548(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169786の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
配列類似性
Belongs to the SPC24 family. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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参照データベース
- Entrez Gene: 147841 Human
- Omim: 609394 Human
- SwissProt: Q8NBT2 Human
- Unigene: 381225 Human
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別名
- FLJ90806 antibody
- hSpc24 antibody
- Kinetochore protein Spc24 antibody
see all
画像
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All lanes : Anti-SPC24 antibody [EPR11548(B)] (ab169786) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : TF1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa -
Detection of SPC24 by Western Blot of Immunprecipitate. 293 cell lysate (1) or 1X PBS (2) immunoprecipitated using ab169786 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab169786 は 7 報の論文で使用されています。
- Uehara DT et al. A missense variant in NUF2, a component of the kinetochore NDC80 complex, causes impaired chromosome segregation and aneuploidy associated with microcephaly and short stature. Hum Genet 140:1047-1060 (2021). PubMed: 33721060
- Wang Y et al. hsa-miR-7-5p suppresses proliferation, migration and promotes apoptosis in hepatocellular carcinoma cell lines by inhibiting SPC24 expression. Biochem Biophys Res Commun 561:80-87 (2021). PubMed: 34020142
- Scott SJ et al. Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments. Cell Death Differ 28:2179-2193 (2021). PubMed: 33649470
- Chen S et al. Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer. Exp Ther Med 22:923 (2021). PubMed: 34306192
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Schleicher K et al. The Ndc80 complex targets Bod1 to human mitotic kinetochores. Open Biol 7:N/A (2017). WB ; Human . PubMed: 29142109
- Zhou J et al. A potential prognostic biomarker SPC24 promotes tumorigenesis and metastasis in lung cancer. Oncotarget 8:65469-65480 (2017). PubMed: 29029446