製品の概要

  • 製品名
    Anti-SPATA4 antibody
    SPATA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SPATA4
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    LILLHMLQRK LGRKLNPEWF DVKPTVGEVT LNHLPAQASG RRYNLKVKRG RVVPVLPNIG SGGSSHREIH VKQAGQHSYY SAMKPIRN, corresponding to C terminal amino acids 213-300 of Human SPATA4 (UniProt ID Q8NEY3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Human testis tissue; RT-4, U251-MG, liver and tonsil lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

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  • Immunocytochemistry/ Immunofluorescence - Anti-SPATA4 antibody (ab121594)
    Immunocytochemistry/ Immunofluorescence - Anti-SPATA4 antibody (ab121594)
    Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121594 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA4 antibody (ab121594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA4 antibody (ab121594)
    ab121594, at a 1/50 dilution, staining SPATA4 in paraffin embedded Human testis tissue by Immunohistochemistry.
  • Western blot - Anti-SPATA4 antibody (ab121594)
    Western blot - Anti-SPATA4 antibody (ab121594)
    All lanes : Anti-SPATA4 antibody (ab121594) at 1/250 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : RT-4 lysate
    Lane 3 : U251-MG lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate

参考文献

ab121594 has not yet been referenced specifically in any publications.

ab121594 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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