Anti-Asef2 抗体 (ab122627)
Key features and details
- Rabbit polyclonal to Asef2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Asef2 antibody
Asef2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Asef2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Asef2 aa 172-281 (internal sequence).
Sequence:ALRPAEWGTLDGSDLEDTDDAFQRSTHRSRSLRRAYGLGRICLLDAPQNH ATPTIATGQVPAVCEILVRDPENNSMGYRRSKSTDNLAFLKKSSFKRKST SNLADLRTAH
-
ポジティブ・コントロール
- Human duodenum tissue; U 251 MG cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122627の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
特記事項 |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
-
機能
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. -
組織特異性
Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
ドメイン
The C-terminal tail is required for its GEF activity. -
細胞内局在
Cytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. - Information by UniProt
-
参照データベース
- Entrez Gene: 221178 Human
- Omim: 613324 Human
- SwissProt: Q96N96 Human
- Unigene: 595391 Human
-
別名
- APC-stimulated guanine nucleotide exchange factor 2 antibody
- ARHGEF29 antibody
- Asef2 antibody
see all
画像
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab122627 は 1 報の論文で使用されています。
- Waseem NH et al. Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma. PLoS Genet 16:e1008721 (2020). PubMed: 32339198