製品の概要

  • 製品名Anti-Spastin antibody [Sp 3G11/1]
    Spastin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Sp 3G11/1] to Spastin
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • WB: Total HeLa extract or rat brain synaptosome This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 52 kDa. There are two splice isoforms of spastin, one without exon4 and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21757506
ICC/IF Use at an assay dependent concentration. PubMed: 21757506

ターゲット情報

  • 関連性Spastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
  • 細胞内局在Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane, Microtubule, Nucleus
  • 参照データベース
  • 別名
    • ADPSP antibody
    • FSP 2 antibody
    • FSP2 antibody
    • KIAA1083 antibody
    • Spast antibody
    • spastic paraplegia 4 (autosomal dominant; spastin) antibody
    • Spastic paraplegia 4 antibody
    • spastin antibody
    • SPG 4 antibody
    • SPG4 antibody
    • SPG4 gene antibody
    see all

Anti-Spastin antibody [Sp 3G11/1] 画像

  • Anti-Spastin antibody [Sp 3G11/1] (ab31850) at 1/500 dilution + rat brain synaptosome

    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • IHC image of Spastin staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31850, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Spastin antibody [Sp 3G11/1] (ab31850) 使用論文

This product has been referenced in:
  • Daftary GS  et al. A Novel Role for the AAA ATPase Spastin as a HOXA10 Transcriptional Corepressor in Ishikawa Endometrial Cells. Mol Endocrinol 25:1539-49 (2011). WB, ICC/IF, IHC-P, ChIP ; Human . Read more (PubMed: 21757506) »
  • Salinas S  et al. Human spastin has multiple microtubule-related functions. J Neurochem 95:1411-20 (2005). Read more (PubMed: 16219033) »

See all 2 Publications for this product

Product Wall

Thank you for your response.

I would expect ab31850 to give clearer results in WB, even once the many isoforms are taken into consideration. I would be happy to offer you a free of charge replacement, credit, or refund for this antibody as we...

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Thank you for your reply.

For the Spastin antibody ab31850, could you please provide an image of your blot or let me know what size bands you are observing? Spastin has several known isoforms that could account for multiple bands between 54-6...

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Thank you for contacting us. I am sorry that these antibodies are not working in WB and IF. We will be happy to refund or replace the antibodies if they are being used in a tested species and application, and have been purchased in the past six months....

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First of all I would like to apologise for the delay in getting back to you. I really appreciate your patience and comprehension.

I contacted my lab colleagues and unfortunately there isn’t any information available about the epitope re...

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