製品の概要

  • 製品名Anti-SPARCL1 antibody
    SPARCL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SPARCL1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 215-244 from the Central region of Human SPARCL1 (NP_001121782.1, NP_004675.3).

  • ポジティブ・コントロール
    • NCI-H460 cell line lysate, Human brain tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab107533 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
  • 配列類似性Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • 翻訳後修飾N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ecm2 antibody
    • Extracellular matrix protein 2 antibody
    • Hevin antibody
    • High endothelial venule protein antibody
    • MAST 9 antibody
    • mast9 antibody
    • Matrix glycoprotein Sc1 antibody
    • PIG33 antibody
    • proliferation inducing protein 33 antibody
    • RAGS1 antigen antibody
    • SC1 antibody
    • SPARC like 1 antibody
    • SPARC-like protein 1 antibody
    • Sparcl1 antibody
    • SPRL1_HUMAN antibody
    see all

Anti-SPARCL1 antibody 画像

  • Anti-SPARCL1 antibody (ab107533) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 75 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107533 at a dilution of 1/50.

Anti-SPARCL1 antibody (ab107533) 使用論文

This product has been referenced in:
  • Hurley PJ  et al. Secreted protein, acidic and rich in cysteine-like 1 (SPARCL1) is down regulated in aggressive prostate cancers and is prognostic for poor clinical outcome. Proc Natl Acad Sci U S A 109:14977-82 (2012). IHC-P . Read more (PubMed: 22927397) »

See 1 Publication for this product

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Thank you for your inquiry.

I am sorry to confirm that we do not expect a cross reactivity of ab107533 with rat.

The immunogen only shows a 52% homology and we only expect a cross reactivity form 85% homology.

I am sorry t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"