Anti-SPARC 抗体 (ab55847)
Key features and details
- Rabbit polyclonal to SPARC
- Suitable for: IHC, ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SPARC antibody
SPARC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SPARC -
由来種
Rabbit -
アプリケーション
適用あり: IHC, ELISA, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from the human SPARC protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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特記事項(精製)
Purified by SAS precipitation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
PubMed: 19509023. |
特記事項 |
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IHC
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. PubMed: 19509023. |
ターゲット情報
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機能
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. -
配列類似性
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
発生段階
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6678 Human
- Entrez Gene: 20692 Mouse
- Omim: 182120 Human
- SwissProt: P09486 Human
- SwissProt: P07214 Mouse
- Unigene: 111779 Human
- Unigene: 708558 Human
- Unigene: 291442 Mouse
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別名
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab55847 は 9 報の論文で使用されています。
- Hatori T et al. SPARC is a decoy counterpart for c‑Fos and is associated with osteoblastic differentiation of bone marrow stromal cells by inhibiting adipogenesis. Mol Med Rep 27:N/A (2023). PubMed: 36633137
- Kalashnikova I et al. Ceria-based nanotheranostic agent for rheumatoid arthritis. Theranostics 10:11863-11880 (2020). PubMed: 33204316
- Xu J et al. Differential Protein Expression between Cystic and Solid Vestibular Schwannoma Using Tandem Mass Tag-Based Quantitative Proteomic Analysis. Proteomics Clin Appl 14:e1900112 (2020). PubMed: 32157794
- Xu J et al. Identification of Predictive Proteins and Biological Pathways for the Tumorigenicity of Vestibular Schwannoma by Proteomic Profiling. Proteomics Clin Appl 13:e1800175 (2019). PubMed: 31120176
- Liu L et al. Secreted Protein Acidic and Rich in Cysteine Mediated Biomimetic Delivery of Methotrexate by Albumin-Based Nanomedicines for Rheumatoid Arthritis Therapy. ACS Nano 13:5036-5048 (2019). PubMed: 30978282
- Wang Q et al. Silencing of SPARC represses heterotopic ossification via inhibition of the MAPK signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31548362
- Farrokhi E et al. Effect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells. Iran Biomed J 19:160-4 (2015). PubMed: 26025968
- Liu Y et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). PubMed: 19403701
- Kos K et al. Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose. Diabetes 58:1780-8 (2009). WB ; Human . PubMed: 19509023