Anti-SPACA3 抗体 (ab121768)
Key features and details
- Rabbit polyclonal to SPACA3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPACA3 antibody -
製品の詳細
Rabbit polyclonal to SPACA3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: TNNGIFQINS RRWCSNLTPN VPNVCRMYCS DLLNPNLKDT VICAMKITQE PQGLGYWEAW RHHCQGKDLT EWVDGCDF, corresponding to amino acids 138-215 of Human SPACA3.
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ポジティブ・コントロール
- Human testis tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121768の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro. -
組織特異性
The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta. -
配列類似性
Belongs to the glycosyl hydrolase 22 family. -
翻訳後修飾
The processed form derives from the membrane form by proteolytic processing. -
細胞内局在
Secreted and Cytoplasmic vesicle > secretory vesicle > acrosome membrane. Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction. - Information by UniProt
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参照データベース
- Entrez Gene: 124912 Human
- Omim: 612749 Human
- SwissProt: Q8IXA5 Human
- Unigene: 434112 Human
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別名
- 1700025M08Rik antibody
- ALLP17 antibody
- Cancer/testis antigen 54 antibody
see all
画像
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ab121768 at a 1/2500 dilution staining SPACA3 in paraffin embedded Human testis tissue by Immunohistochemistry.
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Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121768 は論文での使用が確認できていません。