Anti-Sp7 / Osterix 抗体 - ChIP Grade (ab22552)

製品の概要

  • 製品名Anti-Sp7 / Osterix antibody - ChIP Grade
    Sp7 / Osterix 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sp7 / Osterix - ChIP Grade
  • アプリケーション適用あり: IHC-Fr, ChIP, ICC/IF, WB, IHC-P, IP, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Sp7/ Osterix.

    (Peptide available as ab24390.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal for Immunohistochemistry (FFPE) in Mouse bone and Rat bone.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 20682789
ICC/IF Use at an assay dependent concentration. PubMed: 20682789
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. PubMed: 17510056
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20410296

ターゲット情報

Anti-Sp7 / Osterix antibody - ChIP Grade 画像

  • ab22552 staining Sp7/ Osterix in C2C12 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were stained with ab22552 at a 1/500 dilution followed by goat anti-rabbit IgG conjugated with Alexa Fluor 488 at a 1/400 dilution. Labeling was detected using a Leica TCS SL inverted laser scanning confocal microscope.

    The subcellular localization of Osterx in C2C12 cells was analyzed by immunofluorescence with ab22552 in cells either transiently transfected with short Osterix construct or incubated in medium without serum. Scale bar 10 µm.
  • Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

  • ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

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  • Image courtesy of Human Protein Atlas

    ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

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  • ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.

    See Abreview

Anti-Sp7 / Osterix antibody - ChIP Grade (ab22552) 使用論文

This product has been referenced in:
  • Pang J  et al. ACVR1-Fc suppresses BMP signaling and chondro-osseous differentiation in an in vitro model of Fibrodysplasia ossificans progressiva. Bone 92:29-36 (2016). WB ; Human . Read more (PubMed: 27492611) »
  • Bian Q  et al. Excessive Activation of TGFß by Spinal Instability Causes Vertebral Endplate Sclerosis. Sci Rep 6:27093 (2016). IHC-P ; Mouse . Read more (PubMed: 27256073) »

See all 52 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization No
Specification Bone
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

投稿 Apr 05 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Bone Marrow)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization No
Specification Bone Marrow
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

投稿 Apr 05 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (periodontal ligament)
Specification periodontal ligament
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 17 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (10% gel)
Sample Rat Cell lysate - whole cell (Primary cultured osteoblasts)
Specification Primary cultured osteoblasts
Blocking step Membrane blocking agent (GE Healthcare) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

幸弘 小原

Verified customer

投稿 Jul 24 2014

Application ChIP on chip
Sample Mouse Cell lysate - whole cell (mc3t3-e1)
Specification mc3t3-e1
Type Native ChIP (N-ChIP)
Detection step Microarray
Positive control Osterix transfection in mc3t3-e1 cell line
Negative control not treated
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (mouse cell)
Total protein in input 500 µg
Specification mouse cell
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

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Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I have passes on your details to our distributor (allied scientific). I am currently in contact ...

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I have checked this antibody and it is suitable for use in WB and IHC. It is unfortunate that you did not get satisfactory results and it appears that you may have received a faulty vial. I have forwarded your cas...

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I have had a look at our records, and have found one order (Order number: xxxxxxxxx) placed by our distributor Allied Scientific Products in India. According to this order, it was placed xxxxxxxxx which is 1 year ...

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I am very sorry for the delay in the reply. I have been in contact with our lab regarding your enquiry. It appears that we only test this antibody in IHC .

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