Anti-Sp7 / Osterix 抗体 (ab94744)

製品の概要

  • 製品名Anti-Sp7 / Osterix antibody
    Sp7 / Osterix 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sp7 / Osterix
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ASSLLEEEVH YGSSPLAMLT AACSKFGGSS PLRDSTTLGK AGTKKPYSVG) of Human Sp7/ Osterix (NP_690599).

  • ポジティブ・コントロール
    • Fetal lung lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-Sp7 / Osterix antibody 画像

  • Anti-Sp7 / Osterix antibody (ab94744) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Sp7 / Osterix with ab94744 at 5µg/ml.

Anti-Sp7 / Osterix antibody (ab94744) 使用論文

ab94744 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HUVECs)
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Loading amount 20 µg
Treatment 10ng/ml IL-1 beta and 20uM Inhibitor for 15min
Specification HUVECs
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 May 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 10 µg
Treatment transfected flag-Osterix construct
Immuno-precipitation step Protein A/G
Specification HeLa cells
Username

Natalia Artigas Robles

Verified customer

投稿 Apr 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue (femur)
Specification femur
Username

Abcam user community

Verified customer

投稿 Jan 06 2016

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"