製品の概要

  • 製品名
    Anti-SP6 antibody
  • 製品の詳細
    Rabbit polyclonal to SP6
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 327 - 376 (DHLAKHMKTH EGAKEEAAGA ASGEGKAGGA VEPPGGKGKR EAEGSVAPSN) of Human SP6 (NP_954871).

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-SP6 antibody 画像

  • Anti-SP6 antibody (ab105652) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 40 kDa
  • ab105652 staining SP6 in Rat dental epithelium in tooth germ and hair follicles by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in 0.01M periodic acid, 0.075M lysine, 2% paraformaldehyde and subjected to Heat-mediated antigen retrieval by microwave with 0.01% citric acid prior to blocking with 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 1%BSA/PBS and incubated with the sample for 12 hours. Antibody was detected by an ABC method using HRP-staining.

    See Abreview

Anti-SP6 antibody (ab105652) 使用論文

ab105652 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (dental epithelium in tooth germ and hair follicles)
Specification
dental epithelium in tooth germ and hair follicles
Fixative
0.01Mperiodic acid 0.075M lysine 2% paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: microwaves in 0.01% citric acid
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: ??°C
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投稿 Sep 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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