製品の概要

  • 製品名Anti-SP100 antibody
    SP100 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SP100
  • アプリケーション適用あり: IHC-P, WB, ChIPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SP100.

    (Peptide available as ab44037.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human lysates: HeLa Whole Cell; Ramos Whole Cell; Hodgkin's Lymphoma Whole Cell - tumor tissue; Lymphoma Whole Cell - tumor tissue; MCF7 Whole Cell and ZR-75-1 Nuclear.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
ChIP
  • 追加情報IHC-P: 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see Abreview).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 100 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May play a role in the control of gene expression.
    • 組織特異性Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
    • 配列類似性Contains 2 HMG box DNA-binding domains.
      Contains 1 HSR domain.
      Contains 1 SAND domain.
    • ドメインThe HSR domain is important for the nuclear body targeting as well as for the dimerization.
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • 翻訳後修飾Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
    • 細胞内局在Nucleus > PML body. Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp686E07254 antibody
      • FLJ00340 antibody
      • FLJ34579 antibody
      • Lysp100b antibody
      • Nuclear antigen Sp100 antibody
      • Nuclear autoantigen Sp 100 antibody
      • Nuclear autoantigen Sp-100 antibody
      • Nuclear autoantigen Sp100 antibody
      • Nuclear dot associated Sp100 protein antibody
      • Nuclear dot-associated Sp100 protein antibody
      • SP 100 antibody
      • SP100 antibody
      • SP100 HMG nuclear autoantigen antibody
      • SP100 nuclear antigen antibody
      • SP100_HUMAN antibody
      • Speckled 100 kDa antibody
      see all

    Anti-SP100 antibody 画像

    • All lanes : Anti-SP100 antibody (ab43151) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : Human lymphatic tumor tissue lysate (hodgkin's lymphoma) - total protein (ab29923)
      Lane 4 : Human lymphatic tumor tissue lysate (lymphoma) - total protein (ab30185)
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 6 : ZR751 nuclear extract lysate (ab14916)

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 100 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
    • ab43151 staining SP100 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with  peroxidase  for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.

      See Abreview

    Anti-SP100 antibody (ab43151) 使用論文

    This product has been referenced in:
    • Zebboudj A  et al. Sodium arsenite induces apoptosis and Epstein-Barr virus reactivation in lymphoblastoid cells. Biochimie 107 Pt B:247-56 (2014). Read more (PubMed: 25241256) »
    • Newhart A  et al. Sp100A promotes chromatin decondensation at a cytomegalovirus-promoter-regulated transcription site. Mol Biol Cell 24:1454-68 (2013). ChIP, ICC/IF ; Human . Read more (PubMed: 23485562) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 10000 cells
    Gel Running Conditions Reduced Denaturing (4-12% gradient)
    Sample Human Cell lysate - whole cell (huh7 liver)
    Specification huh7 liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 15 2014

    DISCOUNT CODE: XXXXXXXX Expiration date: 15/03/2012 I am very pleased to hear you would like to accept our offer and test ab43151 in ICC/IF. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS)
    Loading amount 35 µg
    Specification U2OS
    Gel Running Conditions Reduced Denaturing (4-20%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 31 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (lymphoma)
    Specification lymphoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Jul 14 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"