製品の概要

  • 製品名Anti-SP1 antibody - ChIP Grade
    SP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SP1 - ChIP Grade
  • アプリケーション適用あり: IP, WB, IHC-P, ChIPmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
  • 免疫原

    Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa750 and the C-terminus (aa785) of human SP1 using the numbering given in entry XP_028606.4 (GeneID 666).

  • ポジティブ・コントロール
    • IHC: Breast Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 1-4 µg/mg of lysate.
WB 1/2500 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration.
ChIP 1/10.

ターゲット情報

  • 機能Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
  • 組織特異性Up-regulated in adenocarcinomas of the stomach (at protein level).
  • 配列類似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 翻訳後修飾Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739 enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1 from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several C-terminal serine and threonine residues.
    Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter.
    Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by phosphorylation.
    Sumoylated by SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation.
    Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved product is susceptible to degradation.
    O-glycosylated; contains at least 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1 phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma).
  • 細胞内局在Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SP 1 antibody
    • SP1 antibody
    • Sp1 transcription factor antibody
    • SP1_HUMAN antibody
    • Specificity protein 1 antibody
    • Transcription factor Sp1 antibody
    • TSFP 1 antibody
    • TSFP1 antibody
    see all

Anti-SP1 antibody - ChIP Grade 画像

  • ChIP analysis using ab13370 binding SP1 in Human primary macrophage. Cells were cross-linked for 15 minutes with formaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. Protein binding was detected using real-time PCR.

     

    See Abreview

  • ab13370 (2µg/ml) staining SP1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • SP1 was immunoprecipitated from HeLa cells whole cell lysate with ab13370. Anti-SP1 antibody - ChIP Grade (ab13370) used at 6µg per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SP1, ab13370 was used at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling SP1 with ab13370 at 1/5000 dilution. DAB detection.



  • Predicted band size : 90 kDa

    Detection of Human SP1 by Western Blot and Immunoprecipitation.  Samples: A. Whole cell lysate (50 mcg for E; 10 mcg for T) from HEK 293T cells that were mock transfected (E) or transfected with a Sp1 expression construct (T).  B. Whole cell lysate (700 mcg) from HEK 293T cells.  Antibody: A. Affinity purified rabbit anti-Sp1 antibody  (ab13370) used at the indicated concentrations.  B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies (lane 2: ab13405, lane 3: ab13370; lane 1: SP-1 antibody, details unknown) using each antibody at 3.75 mcg/mg lysate.  Subsequently, ab13370 was used at 0.04 mcg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).

Anti-SP1 antibody - ChIP Grade (ab13370) 使用論文

This product has been referenced in:
  • Deng X  et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene N/A:N/A (2016). WB ; Human . Read more (PubMed: 27546619) »
  • Guaraldo M  et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016). ChIP ; Human . Read more (PubMed: 27625068) »

See all 16 Publications for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Human Cell lysate - whole cell (primary human macrophages)
Specification primary human macrophages
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
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投稿 Jan 17 2014

DISCOUNT CODE: 4XXXXX Expiration date: 28-04-2012 I am very pleased to hear you would like to accept our offer and test ab13370 in ChIP on Rat samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pl...

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Application ChIP
Sample Mouse Cell lysate - whole cell (Breast)
Specification Breast
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formalin
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Verified customer

投稿 Feb 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (Breast)
Specification Breast
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formalin
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投稿 Feb 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 1e+006 cells
Specification Breast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Feb 10 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Breast)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 1e+006 cells
Specification Breast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Feb 10 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Intestine tissue)
Gel Running Conditions Reduced Denaturing (7.5% gel SDS)
Loading amount 50 µg
Specification Intestine tissue
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
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Liwei Xie

Verified customer

投稿 Jul 05 2012

DISCOUNT CODE: ***
Expiration date: November 3rd, 2012

I am very pleased to hear you would like to accept our offer and test ab13370 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - nuclear (Intestine tissue)
Loading amount 50 µg
Specification Intestine tissue
Gel Running Conditions Reduced Denaturing (7.5% gel SDS)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Liwei Xie

Verified customer

投稿 Jul 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Rat Cell lysate - nuclear (rat PC12 cell)
Specification rat PC12 cell
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (final conc: 1% )
Detection step Semiquantitative PCR
Positive control In the DNA electrophoretogram, lane 4 is another anti-SP1 Ab (millipore, Cat. 17-601) that reacts with rat.
Negative control In the DNA electrophoretogram, lane2 is anti-rabbit IgG.
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投稿 May 02 2012

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"