製品の概要

  • 製品名
    Anti-SOX9 (phospho S181) antibody
    SOX9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX9 (phospho S181)
  • 特異性
    Detects endogenous levels of SOX9 only when phosphorylated at serine 181.
  • アプリケーション
    適用あり: ELISA, IHC-P, WB, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
  • 免疫原

    Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).

  • ポジティブ・コントロール
    • Human brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 23382206
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • 関連疾患
    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • 配列類似性
    Contains 1 HMG box DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 (phospho S181) antibody 画像

  • All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PBS (60mins)
    Lane 2 : 293 cell extracts treated with PBS (60mins) with immunizing phospho-peptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-SOX9 (phospho S181) antibody (ab59252) 使用論文

This product has been referenced in:
  • Hsu YH  et al. Anti-IL-20 monoclonal antibody inhibited inflammation and protected against cartilage destruction in murine models of osteoarthritis. PLoS One 12:e0175802 (2017). WB ; Human . Read more (PubMed: 28426699) »
  • Suchorska WM  et al. Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs). Stem Cell Rev 13:299-308 (2017). ICC/IF ; Human . Read more (PubMed: 27987073) »

See all 11 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Chondrocyte cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Human Chondrocyte cell line
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1000µg/mL · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 09 2016

Thank you for contacting us and for your interest in our products.

Indeed we can provide discount codes for the value of 100% discount off the next purchase to customers submitting Abreviews for testing our antibodies in new species / applica...

Read More
Application
Western blot
Sample
Cow Cell lysate - whole cell (chondrocytes lysate in RIPA buffer)
Loading amount
20 µg
Specification
chondrocytes lysate in RIPA buffer
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C
Username

Dr. Nazish Ahmed

Verified customer

投稿 Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human cells)
Loading amount
30 µg
Specification
human cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Michaela Leyh

Verified customer

投稿 Feb 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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