製品の概要

  • 製品名Anti-SOX9 antibody
    SOX9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX9
  • 特異性ab71762 recognizes human SOX9 protein without phosphorylation at S181. It does not recognize SOX9 protein phosphorylated at S181.
  • アプリケーション適用あり: EMSA, IHC-P, Dot blot, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Dog, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic non-phosphopeptide (Human) surrounding the epitope RKSVK (amino acids 179 - 183) from SOX9.

  • ポジティブ・コントロール
    • LNCaP prostate Carcinoma whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
EMSA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Dot blot 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

ターゲット情報

  • 機能Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • 関連疾患Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 antibody 画像

  • All lanes : Anti-SOX9 antibody (ab71762) at 1/500 dilution

    Lane 1 : LNCaP prostate Carcinoma whole cell lysate
    Lane 2 : Negative control


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab71762 at 1/1000 dilution staining 1 µg of the following peptides on nitrocellulose membrane:
    A: SOX9 (pS181)
    B: SOX9 (Paired S181) (Nonphospho)
    C: Non-related phosphospecific PP.
  • Immunohistochemical analysis of Eublepharis macularius tail tissue, staining SOX9 with ab71762.

    Sections were blocked with 3% normal goat serum in PBS for 1 hour at room temperature and incubated overnight at 4°C with primary antibody (1/600 in PBS). Staining was detected with DAB.

Anti-SOX9 antibody (ab71762) 使用論文

This product has been referenced in:
  • McLean KE & Vickaryous MK A novel amniote model of epimorphic regeneration: the leopard gecko, Eublepharis macularius. BMC Dev Biol 11:50 (2011). IHC-P . Read more (PubMed: 21846350) »
  • Betancur P  et al. Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest. Proc Natl Acad Sci U S A 107:3570-5 (2010). EMSA . Read more (PubMed: 20139305) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (duodenum)
Specification duodenum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Bond Epitope Retrieval 2
Permeabilization No
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投稿 May 14 2013

Gracias por contactarnos.

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