製品の概要

  • 製品名Anti-SOX9 antibody
    SOX9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX9
  • 特異性Detects endogenous levels of total SOX9 protein.
  • アプリケーション適用あり: WB, IHC-P, ELISA, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).

  • ポジティブ・コントロール
    • Human brain.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab59265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
Flow Cyt Use at an assay dependent concentration. PubMed: 21818404ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • 関連疾患Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 antibody 画像

  • SOX9 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to SOX9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59265.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 56kDa; SOX9

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59265 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide. Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59265 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
  • All lanes : Anti-SOX9 antibody (ab59265) at 1/500 dilution

    Lane 1 : extracts from 293 cells, treated with PBS (60mins)
    Lane 2 : extracts from 293 cells, treated with PBS (60mins) with immunizing non-phosphopeptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa

Anti-SOX9 antibody (ab59265) 使用論文

This product has been referenced in:
  • Kurth TB  et al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum 63:1289-300 (2011). Read more (PubMed: 21538315) »
  • Handorf AM & Li WJ Fibroblast growth factor-2 primes human mesenchymal stem cells for enhanced chondrogenesis. PLoS One 6:e22887 (2011). WB, Flow Cyt ; Human . Read more (PubMed: 21818404) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - 0.3% triton X-100
Blocking step Protein block buffer - Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"