製品の概要

  • 製品名Anti-SOX9 antibody
    SOX9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX9
  • アプリケーション適用あり: WB, Sandwich ELISA, IHC-Pmore details
    適用なし: ICC/IF or IHC-Fr
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Cow, Dog, Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX9.

    (Peptide available as ab27845.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Neuroectoderm (HESCS differentiated) Day 5 and Day 10 - Whole Cell, Caco-2 Whole Cell, SW480 Whole Cell, Mouse Fetus (14 day old) Tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
Sandwich ELISA Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to SOX9 (ab58191). For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal to SOX9 (ab58191) as Capture.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for ICC/IF or IHC-Fr.
  • ターゲット情報

    • 機能Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
    • 関連疾患Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
    • 配列類似性Contains 1 HMG box DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • campomelic dysplasia autosomal sex reversal antibody
      • CMD 1 antibody
      • CMD1 antibody
      • CMPD 1 antibody
      • CMPD1 antibody
      • SOX 9 antibody
      • Sox9 antibody
      • SOX9_HUMAN antibody
      • SRA 1 antibody
      • SRA1 antibody
      • SRXX2 antibody
      • SRXY10 antibody
      • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
      • SRY (sex determining region Y) box 9 antibody
      • SRY (sex determining region Y)-box 9 antibody
      • SRY (sex-determining region Y)-box 9 protein antibody
      • SRY related HMG box gene 9 antibody
      • Transcription factor SOX 9 antibody
      • Transcription factor SOX-9 antibody
      • transcription factor SOX9 antibody
      see all

    Anti-SOX9 antibody 画像

    • All lanes : Anti-SOX9 antibody (ab26414) at 2 µg/ml

      Lane 1 : Neuroectoderm (differentiated from human embryonic stem cells) Day 5 - Whole Cell Lysate
      Lane 2 : Neuroectoderm (differentiated from human embryonic stem cells) Day 10 - Whole Cell Lysate
      Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : Mouse Fetus (14 Day Old) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 56 kDa
      Additional bands at : 100 kDa,90 kDa. We are unsure as to the identity of these extra bands.

      Secondary antibody goat anti-rabbit HRP preadsorbed (IgG H&L; ab97080)

    • Anti-SOX9 antibody (ab26414) at 1 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 56 kDa


      Exposure time : 90 seconds
    • IHC-P image of SOX9 (ab26414) on E11.5 mouse embryo sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Tris/EGTA (pH9). The sections were then blocked in 1% BSA solution for 30 mins at 20°C. In these images ab26414 can be seen staining sections showing spinal cord and developing cartilage (top row), developing cartilage in vertebrae (bottom row).

      See Abreview

    • Standard Curve for SOX9 ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to SOX9 (ab58191) at 5µg/ml and Detector Antibody Rabbit polyclonal to SOX9 (ab26414) at 0.1µg/ml.
    • Immunohistochemical analysis of formalin-fixed, paraffin embedded mouse spleen tissue, staining SOX9 with ab26414.

      Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1.5% serum for 45 minutes 21°C; antigen retrieval was by heat mediation in a Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (0.8 µg/ml in 0.3% Triton X-100 in PBS) for 1 hours at 21°C. An biotin-conjugated goat anti-rabbit polyclonal IgG (7 µg/ml) was used as the secondary antibody.

      A = Primary antibody
      B = No primary antibody

      See Abreview

    プロトコール

    Anti-SOX9 antibody (ab26414) 使用論文

    This product has been referenced in:
    • Liang W  et al. Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods. Exp Ther Med 11:481-489 (2016). IHC-P ; Mouse . Read more (PubMed: 26893634) »
    • Chakkalakal SA  et al. Palovarotene Inhibits Heterotopic Ossification and Maintains Limb Mobility and Growth in Mice With the Human ACVR1(R206H) Fibrodysplasia Ossificans Progressiva (FOP) Mutation. J Bone Miner Res 31:1666-75 (2016). Read more (PubMed: 26896819) »

    See all 15 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (SAOS cells)
    Gel Running Conditions Reduced Denaturing
    Loading amount 25 µg
    Specification SAOS cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    投稿 Oct 27 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Skin)
    Permeabilization No
    Specification Skin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Paraformaldehyde
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    Ahmar Aziz

    Verified customer

    投稿 Apr 05 2016

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (Intestine)
    Specification Intestine
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Feb 12 2014

    Application Western blot
    Loading amount 30000 cells
    Gel Running Conditions Reduced Denaturing (11)
    Sample Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
    Specification MCF-7 cells (human breast adenocarcinoma cell line
    Treatment 0.5% oxygen for 16hrs
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    投稿 Nov 05 2013

    Application Western blot
    Loading amount 30000 cells
    Gel Running Conditions Reduced Denaturing (11)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast (MEF))
    Specification Mouse embryonic fibroblast (MEF)
    Treatment 0.5% oxygen for 6hrs
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    投稿 Nov 05 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample Marmoset (common) Tissue sections (Neonatal testis)
    Specification Neonatal testis
    Permeabilization No
    Fixative Formaldehyde
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    投稿 Oct 31 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Spleen)
    Specification Spleen
    Fixative 10% neutral buffered formalin
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0 (20 min, 98*C)
    Permeabilization No
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
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    投稿 Jan 24 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Articular Cartilage and Aortic Valve Tissue)
    Specification Articular Cartilage and Aortic Valve Tissue
    Fixative 10% NBF
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization No
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

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    Verified customer

    投稿 Jan 24 2013

    Thank you for your enquiry regarding ab26414 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    Though you have kindly provided some details, it ...

    Read More

    Thank you for your enquiry regarding ab26414 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    Regarding specific markers for Sertoli cells, you...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"