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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX9.
(Peptide available as ab27845.)
Our Abpromise guarantee covers the use of ab26414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
|Sandwich ELISA||Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal to SOX9 (ab58191) as Capture.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|