製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SOX6
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Cow, Human
    交差が予測される動物種: Rat, Horse, Non human primates
  • 免疫原

    Synthetic peptide:

    YDDYEDDPKSDYSSENE

    , corresponding to amino acids 780-796 of Human SOX6

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa and Jurkat Nuclear Extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21880783

ターゲット情報

  • 機能
    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • 組織特異性
    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • 配列類似性
    Contains 1 HMG box DNA-binding domain.
  • 翻訳後修飾
    Sumoylation inhibits the transcriptional activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

画像

  • This image shows notochord of 12 d.p.c. mouse embryos stained with ab66316 at 1/100 dilution. Detection was done using anti-rabbit-Cy3 conjugated secondary antibodies (red fluorescence). Cell nuceli were counterstained with DAPI (blue fluorescence).
  • All lanes : Anti-SOX6 antibody (ab66316) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 20 kDa,50 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

参考文献

This product has been referenced in:
  • Ahmed N  et al. Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction. Tissue Eng Part A 20:2224-33 (2014). WB ; Cow . Read more (PubMed: 24606204) »
  • Wang X  et al. Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo. Development 138:4399-404 (2011). IHC-Fr ; Zebrafish . Read more (PubMed: 21880783) »

See all 2 Publications for this product

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