製品の概要

  • 製品名Anti-SOX6 antibody
    SOX6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX6
  • 特異性This antibody has been tested in IHC-P in wildtype post-natal mouse brain sections and showed specific staining. No immunoreactivity was observed in control samples of post-natal brain from a Sox6 knockout mouse.
  • アプリケーション適用あり: IHC-P, IP, WB, IHC - Wholemount, ChIP, IHC-FoFr, CHIPseqmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse SOX6.

    (Peptide available as ab30530.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 21985497
IHC-FoFr 1/300.
CHIPseq Use at an assay dependent concentration. PubMed: 21985497

ターゲット情報

  • 機能Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • 組織特異性Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 翻訳後修飾Sumoylation inhibits the transcriptional activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Anti-SOX6 antibody 画像

  • ab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
  • IHC-FoFr image of Sox6 staining in Rat hippocampus sections using ab30455.The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-SOX6 antibody (ab30455) 使用論文

This product has been referenced in:
  • Qu X  et al. MiR-132-3p Regulates the Osteogenic Differentiation of Thoracic Ligamentum Flavum Cells by Inhibiting Multiple Osteogenesis-Related Genes. Int J Mol Sci 17:N/A (2016). WB ; Human . Read more (PubMed: 27556448) »
  • Darmanis S  et al. A survey of human brain transcriptome diversity at the single cell level. Proc Natl Acad Sci U S A 112:7285-90 (2015). Read more (PubMed: 26060301) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing
Loading amount 15 µg
Specification brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 03 2016

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Tissue lysate - whole (Mouse cortex)
Specification Mouse cortex
Total protein in input 20 µg
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投稿 Dec 31 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Human Tissue lysate - whole (HEK293T cells)
Specification HEK293T cells
Treatment Myc-tagged Sox6 co-transfected with E1, E2 or E3 ligases
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Oct 24 2014

Abreviews
Application ChIP
Detection step Real-time PCR
Sample Mouse Tissue lysate - nuclear (Primary chondrocytes)
Specification Primary chondrocytes
Negative control DNA primers targeting non-binding region
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control DNA primers targeting promoter region
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投稿 Oct 23 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Cell lysate - whole cell (Muscle C2C12 cells)
Specification Muscle C2C12 cells
Treatment 100 μg/ml cycloheximide (CHX)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Oct 23 2014

Application IHC - Wholemount
Sample Mouse Tissue (Mouse limb muscle)
Specification Mouse limb muscle
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投稿 May 02 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Cell (NIH 3T3 fibroblasts)
Specification NIH 3T3 fibroblasts
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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投稿 May 02 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Mouse Tissue sections (Mouse epidermis)
Specification Mouse epidermis
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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投稿 May 02 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Rat Cell lysate - whole cell (C2C12 cell line)
Specification C2C12 cell line
Total protein in input 500 µg
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投稿 Apr 07 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - triton X-100
Fixative Paraformaldehyde
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投稿 Mar 19 2014

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"