製品の概要

  • 製品名Anti-SOX5 antibody
    SOX5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX5
  • アプリケーション適用あり: IHC-Fr, IHC-P, ELISAmore details
  • 種交差性
    交差種: Chicken, Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: PEPGMPVIQS TYGVKGEEPH IKEEIQAEDI NGEIYDEYDE EEDDPDVDYG , corresponding to C terminal amino acids 702-751 of isoform 1, or 316-365 of isoform 3 of Human SOX5(Peptide available as ab104436.)

  • ポジティブ・コントロール
    • HepG2 cell lysate and human lung

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab26041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/500. PubMed: 19521496
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.

ターゲット情報

Anti-SOX5 antibody 画像

  • ab26041 at a concentration of 4-8 µg/ml staining human SOX5 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26041 at 1/500 dilution staining SOX5 in chicken embryo head section by immunohistochemistry (Frozen sections). Sections were incubated overnight in 30% sucrose in PBS at 4°C, embedded in OCT freezing medium, frozen and sectioned in a cryostat. After fixation sections were rehydrated in PBS for 15 min and then blocked in PBS containing 1% fetal calf serum, 0.1% Triton-X and 0.02% Thimerosal. Primary antibody was used overnight at 4°C. The secondary antibody was used at 1/200 dilution for 2 hours.

Anti-SOX5 antibody (ab26041) 使用論文

This product has been referenced in:
  • Aza-Carmona M  et al. NPPB and ACAN, two novel SHOX2 transcription targets implicated in skeletal development. PLoS One 9:e83104 (2014). WB . Read more (PubMed: 24421874) »
  • Fernandes AM  et al. Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage. PLoS One 8:e62994 (2013). IHC-P ; Human . Read more (PubMed: 23671648) »

See all 10 Publications for this product

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Application Western blot
Sample Human Cell lysate - nuclear (progenitor cells)
Loading amount 100 µg
Specification progenitor cells
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Jun 04 2010

Thank you for your enquiry. This antibody was produced outside of Abcam, and the originator considers the exact immunogen sequence proprietary. Thus I cannot give you the exact sequence. The peptide was derived from a region between residues 286 -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"