製品の概要

  • 製品名Anti-SOX4 antibody
    SOX4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX4
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 276-305 of Human SOX4.

  • ポジティブ・コントロール
    • 293, Jurkat, CEM and Y79 cell lysates; Human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 47 kDa.

ターゲット情報

  • 機能Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
  • 組織特異性Testis, brain, and heart.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ecotropic viral integration site 16 antibody
    • EVI 16 antibody
    • EVI16 antibody
    • Sox4 antibody
    • SOX4_HUMAN antibody
    • SRY (sex determining region Y) box 4 antibody
    • SRY box containing gene 4 antibody
    • SRY related HMG box gene 4 antibody
    • Transcription factor SOX-4 antibody
    see all

Anti-SOX4 antibody 画像

  • All lanes : Anti-SOX4 antibody (ab85204) at 1/50 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : CEM cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Y79 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 47 kDa
  • ab85204 at 1/50 dilution staining SOX4 in Formalin-fixed, Paraffin-embedded Human Brain tissue by Immunohistochemistry followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-SOX4 antibody (ab85204) 使用論文

ab85204 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

As requested, I am sending you a vial of ab90696 as a free of charge replacement, since the other SOX4 antibodies you have tried, have not been giving the correct signal in western blotting. Your new order number is *...

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Thank you for your reply.

Please find the answers to your questions below:

1 - Which one should be the SOX4 band?
The band that is above the 40kDa molecular weight is the SOX4 signal. We are unaware of the identity of the lower mo...

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Thank you for your reply.

I am sorry about the continued issues you are having with the SOX4 antibodies. If you are getting the same non-specific bands using both antibodies in your Jurkat cell lysate, it could be that Sox4 antibodies are reco...

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Thank you for contacting Abcam.

I am sorry about the issues you are having with ab85204 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples.

Based ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"