製品の概要

  • 製品名Anti-SOX3 antibody
    SOX3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX3
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    非交差種: Chicken, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Raji cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
  • 関連疾患Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
    Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
    Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GHDX antibody
    • MRGH antibody
    • PHP antibody
    • PHPX antibody
    • sox3 antibody
    • SOX3_HUMAN antibody
    • SOXB antibody
    • SRY (sex determining region Y)-box 3 antibody
    • SRY Box 3 antibody
    • SRY-related HMG-box gene 3 antibody
    • Transcription factor Sox-3 antibody
    see all

Anti-SOX3 antibody 画像

  • Anti-SOX3 antibody (ab42471) at 0.5 µg/ml + Raji cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Imunocytochemistry on primary murine neural stem cell cultures

    Cells were washed with PBS once and fixed in 4%PFA/PBS for 10 min at RT.
    Cells were permeabilized with 0.2% trition X-100/PBS (1ml/each well) for 20 min at RT
    Cells were blocked using 10%BSA/0.1% trition X-100/PBS (1ml/each well) for 30 min at RT
    Ab42471 was added at 1:500 and incubated over night at 4?.
    Cells were washed twice with 0.1% trition X-100/PBS.
    Cells were incubated with secondary Ab (Flourescein anti-mouse) at RT for 1 hour in the dark (1:200), followed by being washed with 0.1% trition X-100/PBS twice
    A: DAPI staining of the nuclei
    B: SOX3 staining using Ab42471
    Image submitted courtesy of: Shih-Han Lee


  • Performed under reducing conditions.

    Predicted band size : 45 kDa

Anti-SOX3 antibody (ab42471) 使用論文

This product has been referenced in:
  • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . Read more (PubMed: 24637840) »

See 1 Publication for this product

Product Wall

Application IHC - Wholemount
Sample Mouse Embryo (E7.5)
Specification E7.5
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投稿 Dec 05 2016

Thank you for your email. Since the peptide sequence is proprietary, we do not have this information about the peptide sequence used to generate this antibody. We can provide you with a 50 amino acid region from which the sequence was derived. This ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"