製品の概要

  • 製品名Anti-SOX3 antibody
    SOX3 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SOX3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide (

    C-DAASPLPGGRLHGVH

    ) corresponding to C terminal amino acids 413-427 of Human SOX3 (NP_005625.2).

  • ポジティブ・コントロール
    • Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
  • 関連疾患Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
    Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
    Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GHDX antibody
    • MRGH antibody
    • PHP antibody
    • PHPX antibody
    • sox3 antibody
    • SOX3_HUMAN antibody
    • SOXB antibody
    • SRY (sex determining region Y)-box 3 antibody
    • SRY Box 3 antibody
    • SRY-related HMG-box gene 3 antibody
    • Transcription factor Sox-3 antibody
    see all

Anti-SOX3 antibody 画像

  • Anti-SOX3 antibody (ab104248) at 0.5 µg/ml + Human Cerebral Cortex lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : ~42 kDa (why is the actual band size different from the predicted?)
  • ab104248 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows nuclear staining at verious intensities along the neuronal cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-SOX3 antibody (ab104248) 使用論文

ab104248 has not yet been referenced specifically in any publications.

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