Anti-SOX2 抗体 (ab137385)
Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SOX2 antibody
SOX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOX2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Cat, Monkey -
免疫原
Recombinant fragment within Human SOX2 (internal sequence). The exact sequence is proprietary.
Database link: P48431 -
ポジティブ・コントロール
- WB: Recombinant Human SOX2 protein (ab79950), NT2D1 whole cell lysate. Human and mouse embryonic stem cell lysate. ICC/IF: Human and mouse embryonic stem cells. A549 cells. IHC-P: Human Cal27 xenograft tissue. Flow Cytometry: Human embryonic stem cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137385の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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ICC/IF | (1) |
1/50 - 1/500.
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特記事項 |
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Flow Cyt
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
ICC/IF
1/50 - 1/500. |
ターゲット情報
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機能
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
関連疾患
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 784383 Cow
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 499593 Rat
- Omim: 184429 Human
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- Unigene: 518438 Human
see all -
別名
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
画像
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Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Human ESC whole cell lysate
at 20 µg
Predicted band size: 34 kDa10% SDS-PAGE gel
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Formalin-fixed, paraffin-embedded Cal27 xenograft tissue stained for SOX2 with ab137385 at a 1/100 dilution in immunohistochemical analysis.
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Human embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
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Flow cytometric analysis of human embryonic stem cells, labelling SOX2 with ab137385 at 1/100 dilution (purple) compared to a rabbit IgG control (black).
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Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 34 kDa12% SDS-PAGE gel
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Anti-SOX2 antibody (ab137385) at 1/3000 dilution + NT2D1 whole cell lysate
at 30 µg
Predicted band size: 34 kDa10% SDS-PAGE gel
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Mouse embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
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Paraformaldehyde-fixed A549 cells stained for SOX2 (green) using ab137385 (1/200 dilution) in ICC/IF. Lower panel is merged with a DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab137385 は 25 報の論文で使用されています。
- Zhang W et al. Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer. Front Oncol 12:805290 (2022). PubMed: 35299743
- Feldheim J et al. Effects of Long-Term Temozolomide Treatment on Glioblastoma and Astrocytoma WHO Grade 4 Stem-like Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35563629
- Liang J et al. Saikosaponin D improves chemosensitivity of glioblastoma by reducing the its stemness maintenance. Biochem Biophys Rep 32:101342 (2022). PubMed: 36186734
- Ceriani R & Whitlock KE Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish. Int J Mol Sci 22:N/A (2021). PubMed: 34072957
- Zhao Y et al. CircVAPA exerts oncogenic property in non-small cell lung cancer by the miR-876-5p/WNT5A axis. J Gene Med 23:e3325 (2021). PubMed: 33619796