製品の概要

  • 製品名Anti-SOX2 antibody [9-9-3]
    SOX2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9-9-3] to SOX2
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Apteronotus leptorhynchus
    交差が予測される動物種: Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human SOX2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HuES7, 46C and IOUD2 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 34 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 関連疾患Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • 翻訳後修飾Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody [9-9-3] 画像

  • Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)

    Target AbID: Ab79351 anti-Sox2, Ab150113 AlexaFluor®488 Goat anti-Mouse secondary

    Counterstain AbID: Ab179504 anti-Tubulin (Rabbit mAb), Ab150080 AlexaFluor®594 Goat anti-Rabbit secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NIH/3T3 cells

    Target primary antibody dilution: 1:200

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:200 (Ab79351)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150080)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab179504)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150113)

  • Cell line: F9 (mouse embryonal carcinoma)

    Target AbID: Ab79351 anti-Sox2, Ab150113 AlexaFluor®488 Goat anti-Mouse secondary

    Counterstain AbID: Ab179504 anti-Tubulin (Rabbit mAb), Ab150080 AlexaFluor®594 Goat anti-Rabbit secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on F9 cells

    Target primary antibody dilution: 1:200

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:200 (Ab79351)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150080)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab179504)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150113)

  • Cell line: NCCIT (human pluripotent embryonal carcinoma)

    Target AbID: Ab79351 anti-Sox2, Ab150113 AlexaFluor®488 Goat anti-Mouse secondary

    Counterstain AbID: Ab179504 anti-Tubulin (Rabbit mAb), Ab150080 AlexaFluor®594 Goat anti-Rabbit secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NCCIT cells

    Target primary antibody dilution: 1:200

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:200 (Ab79351)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150080)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab179504)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150113)

  • All lanes : Anti-SOX2 antibody [9-9-3] (ab79351) at 1/10000 dilution

    Lane 1 : NCCIT whole cell lysate
    Lane 2 : PC-3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • ab79351 staining SOX2 in 16 µm thick sections of Apteronotus leptorhynchus brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab79351 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.

    See Abreview

  • ICC/IF image of ab79351 stained D3 mouse embryonic stem cells. The cells were fixed in 4% Paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT, before incubation with ab79351 at a 1/100 dilution for 2 hours at RT. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse polyclonal, used at 1/200 dilution.

    See Abreview

  • Overlay histogram showing F9 cells stained with ab79351 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79351, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed F9 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-SOX2 antibody [9-9-3] (ab79351) 使用論文

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . Read more (PubMed: 27662313) »
  • Requejo-Aguilar R  et al. PINK1 deficiency sustains cell proliferation by reprogramming glucose metabolism through HIF1. Nat Commun 5:4514 (2014). Read more (PubMed: 25058378) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryonic brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Sodium-citrate buffer, pH 6.0
Permeabilization Yes - 0.3% Triton X-100 in PBS for 10 minutes
Specification embryonic brain
Blocking step 10% goat serum + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Dr. Laura Lahti

Verified customer

投稿 Apr 20 2015

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (4-12% Nupage, MOPS)
Sample Mouse Cell lysate - whole cell (Embryonic stem cells (E14Tg2a))
Specification Embryonic stem cells (E14Tg2a)
Treatment DMSO or GlcNAcstatin C (Oga inhibitor) for 24h
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (12µm frozen sections of mouse cerebellum)
Specification 12µm frozen sections of mouse cerebellum
Fixative Paraformaldehyde
Permeabilization No
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (D3 Mouse Embryonic Stem Cell Line)
Loading amount 200000 cells
Specification D3 Mouse Embryonic Stem Cell Line
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Joe Segal

Verified customer

投稿 Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (D3 (Mouse Embryonic Stem Cell))
Specification D3 (Mouse Embryonic Stem Cell)
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Joe Segal

Verified customer

投稿 Sep 04 2012

Vielen Dank fürIhre Anfrage.

Leider haben wirH00006657-A01 derzeit nicht in unserem Katalog verfügbar. Allerdings kann ich Ihnen gerne drei unserer anti-SOX2- Antikörper empfehlen, die wir für dieImmunhistochemie an humane...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dogfish/Catshark Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 17 2011

Thank you for your call today, and I apologize for the trouble with these codes! I have created new codes which will expire on March 1, 2012. I will go through your Abreviews and put these new codes in our internal notes so that the discounts can be ap...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

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