Anti-SOX2 抗体 [20G5] (ab171380)
Key features and details
- Mouse monoclonal [20G5] to SOX2
- Suitable for: IHC-P, IP, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-SOX2 antibody [20G5]
SOX2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [20G5] to SOX2 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Sheep, Chicken, Xenopus laevis, Zebrafish, Xenopus tropicalis -
免疫原
Recombinant full length protein corresponding to Human SOX2 aa 1-317. Expressed in bacteria.
Database link: 6657 -
ポジティブ・コントロール
- IHC-P: Human lung squamous carcinoma tissue. Mouse esophagus tissue. ICC/IF: H9 embryonic stem cells. HEL 11.4 induced IPS cells. IP: NCCIT whole cell lysate. WB: NCCIT and NTERRA cell lysate. Flow Cytometry: H9 embryonic stem cells. HEL 11.4 induced IPS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 30% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
20G5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171380の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/20 - 1/200.
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IP |
Use at 5 µg/mg of lysate.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 34 kDa.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200.
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特記事項 |
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IHC-P
1/20 - 1/200. |
IP
Use at 5 µg/mg of lysate. |
WB
1/1000 - 1/2000. Predicted molecular weight: 34 kDa. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200. |
ターゲット情報
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機能
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
関連疾患
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 396105 Chicken
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 398000 Xenopus laevis
- Entrez Gene: 378723 Zebrafish
- Omim: 184429 Human
- SwissProt: P48430 Chicken
- SwissProt: P48431 Human
see all -
別名
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
画像
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All lanes : Anti-SOX2 antibody [20G5] (ab171380) at 1/500 dilution
Lane 1 : NCCIT (Human pluripotent embryonic carcinoma cell line) cell lysate
Lane 2 : NTERRA cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa -
REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and SOX2 (using ab171380).
Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections.
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Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated human lung squamous carcinoma (right) compared with a negative control without primary antibody (left).
To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunofluorescence analysis of formaldehyde-fixed H9 embryonic stem cells, labeling SOX2 using ab171380 (left panel) at a 1/200 dilution overnight.
DAPI was used to stain the cell nuclei (central panel). Slides were washed with PBS and incubated with a fluorescein-conjugated secondary antibody at a 1/100 dilution.
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Flow cytometry analysis of H9 embryonic stem cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.
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Immunofluorescence analysis of formaldehyde-fixed HEL 11.4 induced IPS cells, labeling SOX2 using ab171380 (left panel) at a 1/200 dilution overnight. DAPI was used to stain the cell nuclei (central panel). Slides were washed with PBS and incubated with a fluorescein-conjugated secondary antibody at a 1/100 dilution.
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Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated mouse esophagus tissue (right) compared with a negative control without primary antibody (left).
To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Flow cytometry analysis of HEL 11.4 induced IPS cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (48)
ab171380 は 48 報の論文で使用されています。
- Majc B et al. Upregulation of Cathepsin X in Glioblastoma: Interplay with γ-Enolase and the Effects of Selective Cathepsin X Inhibitors. Int J Mol Sci 23:N/A (2022). PubMed: 35163706
- Shen P et al. LncRNA CASC15 regulates breast cancer cell stemness via the miR-654-5p/MEF2D axis. J Biochem Mol Toxicol 36:e23023 (2022). PubMed: 35235236
- Radi R et al. Indolium 1 Exerts Activity against Vemurafenib-Resistant Melanoma In Vivo. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624662
- Zhu H et al. Prognostic Value and Biological Function of Galectins in Malignant Glioma. Front Oncol 12:834307 (2022). PubMed: 35814469
- Deng J et al. Regulation of the TUG1/miR-145-5p/SOX2 axis on the migratory and invasive capabilities of melanoma cells. Exp Ther Med 24:599 (2022). PubMed: 35949341