製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SOX15
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 180-229 (SHCKLEAPSP CSLPQSDPRL QGELLPTYTH YLPPGSPTPY NPPLAGAPMP) of human SOX15 (NP_008873).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

ターゲット情報

  • 機能
    Binds to the 5'-AACAAT-3' sequence.
  • 組織特異性
    Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
  • 配列類似性
    Contains 1 HMG box DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein SOX-12 antibody
    • Protein SOX-15 antibody
    • Protein SOX-20 antibody
    • SOX 12 protein antibody
    • SOX 15 antibody
    • SOX 15 protein antibody
    • SOX 20 protein antibody
    • SOX12 antibody
    • SOX12 protein antibody
    • SOX15 antibody
    • SOX15 protein antibody
    • SOX15_HUMAN antibody
    • SOX20 antibody
    • SOX20 protein antibody
    • SOX26 antibody
    • SOX27 antibody
    • SRY box 15 antibody
    • SRY sex determining region Y box 15 antibody
    • SRY sex determining region Y box 20 antibody
    • SRYbox 15 antibody
    see all

画像

  • Anti-SOX15 antibody (ab82921) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab82921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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