製品の概要

  • 製品名
    Anti-SOX15 antibody
    SOX15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOX15
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: GPLWGPGYAT TQPSRGFGYR PPSYSTAYLP GSYGSSHCKL EAPSPCSLPQ, corresponding to internal sequence amino acids 145-194 of Human SOX15

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Transfected 293T cell lysate; human kidney tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Binds to the 5'-AACAAT-3' sequence.
  • 組織特異性
    Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
  • 配列類似性
    Contains 1 HMG box DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Protein SOX-12 antibody
      • Protein SOX-15 antibody
      • Protein SOX-20 antibody
      • SOX 12 protein antibody
      • SOX 15 antibody
      • SOX 15 protein antibody
      • SOX 20 protein antibody
      • SOX12 antibody
      • SOX12 protein antibody
      • SOX15 antibody
      • SOX15 protein antibody
      • SOX15_HUMAN antibody
      • SOX20 antibody
      • SOX20 protein antibody
      • SOX26 antibody
      • SOX27 antibody
      • SRY box 15 antibody
      • SRY sex determining region Y box 15 antibody
      • SRY sex determining region Y box 20 antibody
      • SRYbox 15 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX15 antibody (ab55960)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX15 antibody (ab55960)

      Immunohistochemistry of (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SOX15 with ab55960 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubules. Magnification: 400X.

    • Western blot - Anti-SOX15 antibody (ab55960)
      Western blot - Anti-SOX15 antibody (ab55960)
      Anti-SOX15 antibody (ab55960) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG at 1/50000 dilution

      Predicted band size: 28 kDa



      Gel concentration 12%

    参考文献

    This product has been referenced in:
    • Rui X  et al. SOX15 regulates proliferation and migration of endometrial cancer cells. Biosci Rep 37:N/A (2017). Read more (PubMed: 28821564) »
    • Proestling K  et al. Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients. Reprod Biol Endocrinol 14:81 (2016). IHC-P ; Human . Read more (PubMed: 27881125) »

    See all 2 Publications for this product

    ab55960 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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