製品の概要

  • 製品名
    Anti-SOX14 antibody
  • 製品の詳細
    Rabbit polyclonal to SOX14
  • 特異性
    This antibody is specific for SOX14
  • アプリケーション
    適用あり: ELISA, WB, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    A region within synthetic peptide: SSSAIQKMGE VPHTLATGAL PYASTLGYQN GAFGSLSCPS QHTHTHPSPT, corresponding to internal sequence amino acids 145-194 of Human SOX14

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC Use at an assay dependent concentration. PubMed: 24637840

ターゲット情報

  • 関連性
    The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate (specifically neuronal). SOX14 is most closely related to SOX factors 1,2 and 3 and likely mirrors expression of these genes in the developing brain and spinal cord.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • HMG box transcription factor SOX 14 antibody
    • MGC119898 antibody
    • MGC119899 antibody
    • SOX 14 antibody
    • SOX 28 antibody
    • Sox box protein 14 antibody
    • SOX28 antibody
    • SRY (sex determining region Y) box 14 antibody
    • SRY box 14 antibody
    • Transcription factor SOX 14 antibody
    see all

画像

  • Anti-SOX14 antibody (ab49047) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa

参考文献

This product has been referenced in:
  • Li F  et al. SOX14 promotes proliferation and invasion of cervical cancer cells through Wnt/ß-catenin pathway. Int J Clin Exp Pathol 8:1698-704 (2015). Read more (PubMed: 25973056) »
  • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB, ICC ; Human . Read more (PubMed: 24637840) »

See all 3 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Formaldehyde
Permeabilization
Yes - PBS-T
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Oct 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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