The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration. PubMed: 24637840
The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate (specifically neuronal). SOX14 is most closely related to SOX factors 1,2 and 3 and likely mirrors expression of these genes in the developing brain and spinal cord.
Li F et al. SOX14 promotes proliferation and invasion of cervical cancer cells through Wnt/ß-catenin pathway. Int J Clin Exp Pathol8:1698-704 (2015).
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Popovic J et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One9:e91852 (2014).
Read more (PubMed: 24637840) »