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Our Abpromise guarantee covers the use of ab108408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue, staining SOX10 with ab108408 at 4 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue, staining SOX10 with ab108408 at 4 µg/ml.