製品の概要

  • 製品名Anti-SOSTDC1 antibody
    SOSTDC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOSTDC1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human SOSTDC1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates. It also gave a positive signal in FFPE human normal lung tissue sections

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction Enhances Wnt signaling and inhibits TGF-beta signaling (By similarity). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.
  • 組織特異性Highly expressed in kidney and weakly in lung.
  • 配列類似性Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDA019 antibody
    • DKFZp564D206 antibody
    • Ectodermal BMP inhibitor antibody
    • Ectodin antibody
    • sclerostin domain containing 1 antibody
    • Sclerostin domain containing protein 1 antibody
    • Sclerostin domain-containing protein 1 antibody
    • SOSD1_HUMAN antibody
    • Sostdc1 antibody
    • USAG-1 antibody
    • USAG1 antibody
    • Uterine sensitization associated gene 1 protein antibody
    • Uterine sensitization-associated gene 1 protein antibody
    • Wise antibody
    see all

Anti-SOSTDC1 antibody 画像

  • All lanes : Anti-SOSTDC1 antibody (ab99340) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab99340 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.
  • IHC image of SOSTDC1 staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99340, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SOSTDC1 antibody (ab99340) 使用論文

This product has been referenced in:
  • Yuan S  et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet 11:e1005253 (2015). Read more (PubMed: 26020521) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - tritonX100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 05 2012

Gracias por enviarnos el Abreview.

Esta mañana lo he publicado en nuestra página web, con las imágenes incluidas. Ha quedado muy bien el montaje, gracias por molestarte.

El código puedes utilizarlo ya. Re...

Read More

¡Gracias a ti!

No olvides una vez enviados tus resultados a nuestra web, hacer el nuevo pedido indicando el código promocional para obtener un 100% de descuento.

Si necesitas mas información no dudes en contactar...

Read More

Gracias por tu respuesta.

Sí que sería válido para testarlo en immunohistoquímica. Para poder mandarte el código es necesario indicar también si las secciones a teñir están congeladas o ...

Read More

Thank you for your interest in our Abreview service. Let me explain how it works:

If you want to test any of the antibodies in an application / species not yet tested, follow these steps:

1. Reply to this e-mail to let me know that ...

Read More

Puede ser cualquier otro anticuerpo de nuestro catálogo.

Te mando el link a nuestro catálogo de posters de rutas. Puedes descargártelo e imprimirlo, o si prefieres te lo podemos mandar por correo postal tamaño post...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"