製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Sortilin
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, ELISA, Dot blotmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Extracellular domain of Human sortilin.

アプリケーション

Our Abpromise guarantee covers the use of ab36959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 5 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/1000.
ELISA 1/5000.
Dot blot 1/2000.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
    • 組織特異性
      Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
    • 関連疾患
      Note=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.
    • 配列類似性
      Belongs to the VPS10-related sortilin family. SORT1 subfamily.
      Contains 9 BNR repeats.
    • ドメイン
      The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
      The extracellular domain may be shed following protease cleavage in some cell types.
    • 翻訳後修飾
      The N-terminal propeptide is cleaved by furin and possibly other homologous proteases.
    • 細胞内局在
      Membrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 100 kDa NT receptor antibody
      • Glycoprotein 95 antibody
      • Gp 95 antibody
      • Gp95 antibody
      • LDLCQ6 antibody
      • Neurotensin receptor 3 antibody
      • NT 3 antibody
      • NT3 antibody
      • NTR 3 antibody
      • NTR3 antibody
      • OTTHUMP00000013784 antibody
      • SORT 1 antibody
      • SORT_HUMAN antibody
      • SORT1 (gene name) antibody
      • Sort1 antibody
      • Sortilin 1 antibody
      • Sortilin antibody
      see all

    画像

    • ab36959 staining Sortilin in Human primary monocytes and macrophages by Flow Cytometry. The sample was incubated with the primary antibody (5µg/ml in DPBS + 2% FBS and 0.1% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 647 -conjugated goat anti-rabbit IgG polyclonal (5µg/ml) was used as the secondary antibody.
      Gating Strategy: Either monocytes or macrophages.

      Red - sortilin, blue - control IgG.

      See Abreview

    参考文献

    ab36959 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Flow Cytometry
    Fixation
    live cells
    Permeabilization
    No
    Sample
    Human Cell (primary monocytes and macrophages)
    Specification
    primary monocytes and macrophages
    Gating Strategy
    either monocytes or macrophages
    Preparation
    Cell harvesting/tissue preparation method: isolated from human peripheral blood
    Sample buffer: DPBS + 2%FBS + 0.1% sodium azide
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    投稿 Jan 14 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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