製品の概要

  • 製品名Anti-SORBS1 antibody
    SORBS1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SORBS1
  • 特異性This antibody is expected to recognise both human isoforms.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide: GTFPGNYVKPLYL, corresponding to C terminal amino acids 804-816 of Human SORBS1.

  • 特記事項GenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab4551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Can be blocked with Human SORBS1 peptide (ab18388). No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
  • 組織特異性Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
  • 配列類似性Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c-Cbl-associated protein antibody
    • CAP antibody
    • CBL Associated Protein antibody
    • Ponsin antibody
    • SH3 domain protein 5 antibody
    • SH3D5 antibody
    • SH3P12 antibody
    • Sorbin and SH3 Domain Containing 1 antibody
    • Sorbin and SH3 domain-containing protein 1 antibody
    • Sorbs1 antibody
    • SRBS1_HUMAN antibody
    see all

Anti-SORBS1 antibody 画像

  • All lanes : Anti-SORBS1 antibody (ab4551)

    Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
    Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
    Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
    Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
    Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
    Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

    Secondary
    Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution
  • ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.

Anti-SORBS1 antibody (ab4551) 使用論文

ab4551 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (white blood cells)
Loading amount 40 µg
Specification white blood cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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投稿 Aug 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"