The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Sorbitol Dehydrogenase is approximately 0.1ng/ml as a capture antibody.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
組織特異性Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
配列類似性Belongs to the zinc-containing alcohol dehydrogenase family.
細胞内局在Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.