製品の概要

  • 製品名Anti-Sonic Hedgehog antibody [RM0128-4A37]
    Sonic Hedgehog 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RM0128-4A37] to Sonic Hedgehog
  • 特異性ab86462 detects Sonic Hedgehog, but not dhh or ihh.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant fragment from the C terminal region of mouse Sonic Hedgehog

  • 特記事項


    ab86462 was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • 組織特異性Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • 関連疾患Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • 配列類似性Belongs to the hedgehog family.
  • 翻訳後修飾The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • 細胞内局在Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HHG 1 antibody
    • HHG-1 antibody
    • HHG1 antibody
    • HLP 3 antibody
    • HLP3 antibody
    • Holoprosencephaly 3 antibody
    • HPE 3 antibody
    • HPE3 antibody
    • MCOPCB5 antibody
    • shh antibody
    • SHH_HUMAN antibody
    • SMMC I antibody
    • SMMCI antibody
    • Sonic Hedgehog (Drosophila) homolog antibody
    • sonic hedgehog homolog (Drosophila) antibody
    • Sonic hedgehog homolog antibody
    • Sonic hedgehog protein antibody
    • Sonic hedgehog protein C-product antibody
    • TPT antibody
    • TPTPS antibody
    see all

Anti-Sonic Hedgehog antibody [RM0128-4A37] 画像

  • ab86462 staining Sonic Hedgehog on an E11.5 mouse embryo by immunohistochemistry (left). The tissue was paraformaldehyde fixed and blocked in 1% BSA for 30 mins at RT. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor 488 conjugated donkey anti-rat antibody was used as the secondary. DAPI was used to stain the nuclei (right).

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Anti-Sonic Hedgehog antibody [RM0128-4A37] (ab86462) 使用論文

This product has been referenced in:
  • Lettice LA  et al. Development of five digits is controlled by a bipartite long-range cis-regulator. Development 141:1715-25 (2014). IHC-P ; Mouse . Read more (PubMed: 24715461) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E11.5 embryo)
Specification E11.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH9, EGTA
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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投稿 Jul 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"