Anti-Sonic Hedgehog 抗体 (ab114122)


  • 製品名Anti-Sonic Hedgehog antibody
    Sonic Hedgehog 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sonic Hedgehog
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Human, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Sonic Hedgehog.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Mouse tissue lysates: E10 Embryonic Brain; E10 Whole Embryo; E12 Embryonic Brain and Spinal Cord; E14 Embryonic Brain; E14 Embryonic Spinal Cord; E16 Embryonic Brain.



Our Abpromise guarantee covers the use of ab114122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).


  • 機能Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • 組織特異性Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • 関連疾患Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • 配列類似性Belongs to the hedgehog family.
  • 翻訳後修飾The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • 細胞内局在Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HHG 1 antibody
    • HHG-1 antibody
    • HHG1 antibody
    • HLP 3 antibody
    • HLP3 antibody
    • Holoprosencephaly 3 antibody
    • HPE 3 antibody
    • HPE3 antibody
    • MCOPCB5 antibody
    • shh antibody
    • SHH_HUMAN antibody
    • SMMC I antibody
    • SMMCI antibody
    • Sonic Hedgehog (Drosophila) homolog antibody
    • sonic hedgehog homolog (Drosophila) antibody
    • Sonic hedgehog homolog antibody
    • Sonic hedgehog protein antibody
    • Sonic hedgehog protein C-product antibody
    • TPT antibody
    • TPTPS antibody
    see all

Anti-Sonic Hedgehog antibody 画像

  • All lanes : Anti-Sonic Hedgehog antibody (ab114122) at 1 µg/ml

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E10 Mouse Embryo Tissue Lysate
    Lane 3 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
    Lane 4 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 5 : E14 Mouse Embryo Spinal Cord Tissue Lysate
    Lane 6 : E16 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 160 kDa,185 kDa,26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-Sonic Hedgehog antibody (ab114122) 使用論文

ab114122 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E10.5 embryo)
Specification E10.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EGTA
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

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投稿 Sep 14 2012