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Purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).
Our Abpromise guarantee covers the use of ab50515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab50515 staining Sonic Hedgehog in human Panc-1 cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was used at 25 μg/ml and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal to rat Ig, diluted 1/700, was used as the secondary antibody.