Anti-Sonic Hedgehog 抗体 [171018] (ab50515)

製品の概要

  • 製品名Anti-Sonic Hedgehog antibody [171018]
    Sonic Hedgehog 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [171018] to Sonic Hedgehog
  • 特異性ab50515 recognizes N-terminal peptide. In immunoblotting, the antibody show approximately 50% crossreactivity with the N-terminal peptide from recombinant mouse Desert hedgehog (Dhh) (amino acids 23-198). The antibody shows no cross-reactivity with recombinant mouse Indian hedgehog (Ihh) (amino acid 66- 240) and no cross-reactivity with the C-terminal peptides of mouse Dhh (amino acids 199-396) or mouse Shh (amino acid 199-437).
  • アプリケーション適用あり: IHC-FoFr, WB, IHC-Fr, Neutralising, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).

  • ポジティブ・コントロール
    • Frozen mouse embryo sections.
  • 特記事項Endotoxin: < 0.1 EU (endotoxin units)/µg antibody as determined by the LAL (Limulus amebocyte lysate) method.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/250. PubMed: 18777197
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
IHC-Fr Use a concentration of 25 µg/ml.
Neutralising Use at an assay dependent concentration.
ELISA Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 25 µg/ml.

ターゲット情報

  • 機能Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • 組織特異性Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • 関連疾患Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • 配列類似性Belongs to the hedgehog family.
  • 翻訳後修飾The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • 細胞内局在Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HHG 1 antibody
    • HHG-1 antibody
    • HHG1 antibody
    • HLP 3 antibody
    • HLP3 antibody
    • Holoprosencephaly 3 antibody
    • HPE 3 antibody
    • HPE3 antibody
    • MCOPCB5 antibody
    • shh antibody
    • SHH_HUMAN antibody
    • SMMC I antibody
    • SMMCI antibody
    • Sonic Hedgehog (Drosophila) homolog antibody
    • sonic hedgehog homolog (Drosophila) antibody
    • Sonic hedgehog homolog antibody
    • Sonic hedgehog protein antibody
    • Sonic hedgehog protein C-product antibody
    • TPT antibody
    • TPTPS antibody
    see all

Anti-Sonic Hedgehog antibody [171018] 画像

  • ab50515 staining Sonic Hedgehog in human Panc-1 cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was used at 25 μg/ml and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal to rat Ig, diluted 1/700, was used as the secondary antibody.

    See Abreview

Anti-Sonic Hedgehog antibody [171018] (ab50515) 使用論文

This product has been referenced in:
  • Smirnova NF  et al. Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs. Respir Res 17:83 (2016). IHC-P ; Human . Read more (PubMed: 27423691) »
  • Nguyen H  et al. Glial scaffold required for cerebellar granule cell migration is dependent on dystroglycan function as a receptor for basement membrane proteins. Acta Neuropathol Commun 1:58 (2013). Mouse . Read more (PubMed: 24252195) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E11.5 embryo)
Specification E11.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH9, EGTA
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 24 2012

I am sorry, but it has not been determined which type of light chain this antibody contains. Sorry we could not be of further assistance in this instance. Please contact us again if you have any further questions.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 31 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E13.5 wildtype embryonic midbrains, 5 microns)
Specification E13.5 wildtype embryonic midbrains, 5 microns
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1 (stable transfectant expressing Shh))
Specification Panc-1 (stable transfectant expressing Shh)
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Jennifer Dembinski

Verified customer

投稿 Sep 03 2009

Thank you for your enquiry. I am afraid that no further information about the epitope has been determined, apart from that already highlighted in the datasheet (immunogen used was aa 25-198). I am sorry I can't be of more assistance in this occ...

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