Anti-Somatostatin Receptor 5 抗体 [UMB4] (ab109495)

製品の概要

  • 製品名Anti-Somatostatin Receptor 5 antibody [UMB4]
    Somatostatin Receptor 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human Somatostatin Receptor 5.

  • ポジティブ・コントロール
    • Human fetal heart and HT29 cell lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Panc-1
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    • 組織特異性Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
    • 配列類似性Belongs to the G-protein coupled receptor 1 family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • SSTR5 antibody
      • Somatostatin receptor subtype 5 antibody
      • Somatostatin receptor type 5 antibody
      • SS 5R antibody
      • SS-5-R antibody
      • SS5 R antibody
      • SS5-R antibody
      • SS5R antibody
      • SSR5_HUMAN antibody
      • SST R5 antibody
      • SSTR 5 antibody
      • Sstr5 antibody
      see all

    Anti-Somatostatin Receptor 5 antibody [UMB4] 画像

    • ICC/IF image of ab109495 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109495 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa

    Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) 使用論文

    This product has been referenced in:
    • Pivonello C  et al. Effects of the single and combined treatment with dopamine agonist, somatostatin analog and mTOR inhibitors in a human lung carcinoid cell line: an in vitro study. Endocrine N/A:N/A (2016). Read more (PubMed: 27688013) »
    • Lehmann A  et al. Carboxyl-terminal receptor domains control the differential dephosphorylation of somatostatin receptors by protein phosphatase 1 isoforms. PLoS One 9:e91526 (2014). Human . Read more (PubMed: 24637622) »

    See all 5 Publications for this product

    Product Wall


    J’ai contacté le laboratoire qui a accepté d’ajouter le peptide bloquant d’ab109495 au catalogue sous la référence ab178801.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"