Anti-Somatostatin Receptor 4 抗体 (ab28578)

製品の概要

  • 製品名Anti-Somatostatin Receptor 4 antibody
    Somatostatin Receptor 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Somatostatin Receptor 4
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    (C)QQEALQPEPGRKRIPLTRTTTF

    , corresponding to C terminal amino acids 366-388 of Human Somatostatin Receptor 4

  • ポジティブ・コントロール
    • ICC/IF: SKNSH cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/2500.
WB 1/100 - 1/5000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/10 - 1/200.

ターゲット情報

  • 機能Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.
  • 組織特異性Specifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Somatostatin receptor type 4 antibody
    • SS 4R antibody
    • SS-4-R antibody
    • SS4-R antibody
    • SS4R antibody
    • SSR4_HUMAN antibody
    • SST R4 antibody
    • SSTR 4 antibody
    • Sstr4 antibody
    see all

Anti-Somatostatin Receptor 4 antibody 画像

  • Immunofluorescent analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Somatostatin Receptor 4 polyclonal antibody ab28578) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Somatostatin Receptor 4 polyclonal antibody (ab285781) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ICC/IF image of ab28578 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28578, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Somatostatin Receptor 4 antibody (ab28578) 使用論文

ab28578 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step NDS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0,3% Trion X100
Fixative Paraformaldehyde
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投稿 Nov 25 2013

Thank you for contacting us. ab28578 has been documented in IHC-P applications with human pheochromocytoma and human gastric mucosa sections. SSTR4 has been reported to be expressed in human brain, gastrointestinal tract, pancreas, and prostate tis...

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