Anti-Somatostatin Receptor 1 抗体 (ab2366)

製品の概要

  • 製品名
    Anti-Somatostatin Receptor 1 antibody
    Somatostatin Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Somatostatin Receptor 1
  • 特異性
    This antibody reacts with a 60 kD protein, known as Somatostatin Receptor 1.
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Rat)A synthetic peptide derived from the C-terminus of rat somatostatin receptor 1

  • ポジティブ・コントロール
    • Pancreas
  • 特記事項


    The Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    The Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/75.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Receptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins.
  • 組織特異性
    Fetal kidney, fetal liver, and adult pancreas, brain, lung, jejunum and stomach.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G-protein coupled receptor antibody
    • Somatostatin R1 antibody
    • Somatostatin receptor 1 antibody
    • Somatostatin receptor isoform 1 antibody
    • Somatostatin receptor type 1 antibody
    • SRIF 2 antibody
    • SRIF-2 antibody
    • SRIF2 antibody
    • SS-1-R antibody
    • SS1 R antibody
    • SS1-R antibody
    • SS1R antibody
    • SSR1_HUMAN antibody
    • SSTR 1 antibody
    • Sstr1 antibody
    see all

画像

  • Formalin fixed paraffin embedded human pancreas stained with Somatostatin Receptor using ABC and AEC chromogen.
  • All lanes : Anti-Somatostatin Receptor 1 antibody (ab2366) at 1 µg/ml

    Lane 1 : PANC-1 nuclear extract lysate (ab14882)
    Lane 2 : Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab2366 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2366, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Kim HS  et al. Clinical significance of protein expression of cyclooxygenase-2 and somatostatin receptors in gastroenteropancreatic neuroendocrine tumors. Cancer Res Treat 43:181-8 (2011). IHC . Read more (PubMed: 22022296) »
  • Fasciani A  et al. Overexpression and functional relevance of somatostatin receptor-1, -2, and -5 in endometrium and endometriotic lesions. J Clin Endocrinol Metab 95:5315-9 (2010). IHC-P ; Human . Read more (PubMed: 20739383) »

See all 2 Publications for this product

レビューと Q&A

This antibody is directed against somatostatin receptor 1.

Gracias por contactarnos.

La concentración de Ig de este anticuerpo es de 66.6ug/ml. Las diluciones óptimas varían en el rango entre 1:40 y 1:400.

Si necesitas consultar nuestros protocolos online, te adjunto el...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Jun 29 2011

I'm sorry to hear you are experiencing problems with ab2366 in immunocytochemistry. This antibody has not been tested in this application, therefore I am unable to provide a specific protocol for this staining method, but I after consultation with ...

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Thank you for your enquiry. This antibody was produced in-house. The paper by Schulz listed on the data sheet, is only a general reference concerning the receptor. If you require further information, please contact us again and we will gladly assist you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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