製品の概要

  • 製品名
    Anti-Somatostatin antibody
    Somatostatin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Somatostatin
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Somatostatin aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P61278
    (Peptide available as ab131563)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human normal pancreas formalin-fixed, paraffin-embedded tissue section within Immunohistochemistry as well as a positive signal in Mouse Placenta tissue lysate and JEG3 whole cell lysate within Western Blot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14, 15 kDa (predicted molecular weight: 13 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • IHC image of Somatostatin staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108456, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Somatostatin antibody (ab108456) at 1 µg/ml

    Lane 1 : Mouse Placenta Tissue Lysate
    Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 14,15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

参考文献

This product has been referenced in:
  • Alfonsi G & Crippa M Updates on the Methodological Approaches for Carrying Out an In-Depth Study of the Cardiac Conduction System and the Autonomic Nervous System of Victims of Sudden Unexplained Fetal and Infant Death. Front Med (Lausanne) 3:54 (2016). Read more (PubMed: 27917382) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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