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RabMAb

Anti-Somatostatin 28 抗体 [EPR3359(2)] (ab111912)

製品の概要

  • 製品名Anti-Somatostatin 28 antibody [EPR3359(2)]
    Somatostatin 28 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3359(2)] to Somatostatin 28
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
    適用なし: Flow Cyt,ICC,IP or WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Somatostatin 28.

  • ポジティブ・コントロール
    • Human pancreas tissue
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IP or WB.
  • ターゲット情報

    Anti-Somatostatin 28 antibody [EPR3359(2)] 画像

    • ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • Ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).  Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID: 25469233] . 

    • ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1:4000 dilution and   a  Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

       

       

    • Ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] .  

    • Ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI.  Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID: 25469233]. 

    • Ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    • ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (ab93684).Primary antibody at 1:4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32]  .

       

       

    • ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody  at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID: 25019573], [PMID: 9066918],  [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912) 使用論文

    This product has been referenced in:
    • Simonet JC  et al. Conditional Loss of Arx From the Developing Dorsal Telencephalon Results in Behavioral Phenotypes Resembling Mild Human ARX Mutations. Cereb Cortex N/A:N/A (2014). Mouse . Read more (PubMed: 24794919) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"