Anti-Soluble TNF Receptor II 抗体 (ab109853)
Key features and details
- Rabbit polyclonal to Soluble TNF Receptor II
- Suitable for: Sandwich ELISA, Neutralising, Indirect ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Soluble TNF Receptor II antibody
Soluble TNF Receptor II 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Soluble TNF Receptor II -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, Neutralising, Indirect ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Highly pure (>98%) recombinant Human Soluble TNF Receptor II.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab109853 was purified by affinity chromatography employing immobilized Soluble TNF Receptor II matrix. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to Soluble TNF Receptor II (ab83158).
(using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab83158 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. |
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Neutralising |
Use at an assay dependent concentration.
to yield one half maximal inhibition [ND50] of the biological activity of Human soluble TNF Receptor II (0.5 µg/ml). |
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Indirect ELISA |
Use at an assay dependent concentration.
(using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. |
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to Soluble TNF Receptor II (ab83158). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab83158 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. |
Neutralising
Use at an assay dependent concentration. to yield one half maximal inhibition [ND50] of the biological activity of Human soluble TNF Receptor II (0.5 µg/ml). |
Indirect ELISA
Use at an assay dependent concentration. (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human soluble TNF Receptor II. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
配列類似性
Contains 4 TNFR-Cys repeats. -
翻訳後修飾
Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7133 Human
- Omim: 191191 Human
- SwissProt: P20333 Human
- Unigene: 256278 Human
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別名
- CD120b antibody
- p75 antibody
- p75 TNF receptor antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109853 は論文での使用が確認できていません。