The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
This working dilution was determined using recombinant human IL2 Rg at 100 ng/lane under non-reducing conditions.
Use a concentration of 2 - 6 µg/ml.
This concentration was found to block 50% of the bioactivity due to 10 ng/ml recombinant human IL2 in a 3H-thymidine incorporation assay using 105 cells/ml MO7e cells.
Use a concentration of 0.5 - 1 µg/ml.
I-ELISA: Use at a concentration of 0.5 - 1 µg/ml (to a limit of 3 ng/well).
関連性A common subunit for the receptors for a variety of interleukins, the gamma chain is common to the IL2, IL4, IL7, IL21 and probably also the IL13 receptors. Defects in IL2RG are the cause of X-linked severe combined immunodeficiency. The biological effects of IL2R signals are much more complex than simply mediating T-cell growth. Depending on conditions, IL2R signals may also promote cell survival, effector function, and apoptosis. These sometimes contradictory effects underscore the fact that a diversity of intracellular signalling pathways are potentially activated by IL2R. There are at least 3 components of the IL2 receptor; IL2R alpha, IL2R beta, and IL2R gamma chains.
細胞内局在Membrane; single-pass type I membrane protein